Hyperbranched chemoselective silicon-based polymers for chemical sensor applications

ABSTRACT

The invention provides a device for selective molecular recognition, the device comprising a sensing portion, wherein said sensing portion includes a substrate having coated thereon a layer comprising a hyperbranched compound having:
         (1) a polymer backbone portion that is at least partly randomly branched;   (2) at least one pendant group extending from the polymer backbone portion; and   (3) at least one halogen substituted alcohol or phenol group substituted at the pendant group(s) of the polymer backbone portion.       

     The compound of the invention preferably has the general formula: 
                         
wherein A is the hyperbranched backbone portion of the polymer;
         L and M are independently selected pendant groups of said polymer backbone;   X and Y are independently selected halogen substituted alcohol or phenol groups;   q and r are independently selected and at least 1; and   n is at least 3.

This is a divisional application of copending application Ser. No.10/091,024 filed on Mar. 6, 2002. The entire contents of applicationSer. No. 10/091,024 are incorporated herein by reference.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to the detection of noxious chemicalspecies by means of chemoselective hyperbranched polymeric compounds.More particularly, the invention relates to the detection of toxic orexplosive chemical vapors, such as chemical agents or nitro-substitutedspecies, respectively, by sorbent materials comprising chemoselectivehyperbranched polymeric molecules.

2. Description of Related Art

Determining and/or monitoring the presence of certain chemical specieswithin an environment, e.g., pollutants, toxic substances and otherpredetermined compounds, is becoming of increasing importance withrespect to such fields as health, environmental protection, resourceconservation, and chemical processes. Devices for the molecularrecognition of noxious species or other analytes typically include (1) asubstrate and (2) a molecular recognition coating upon the substrate.These devices may be used, for example, in chemical vapor sensing or theselective separation of gases by gas chromatography. Small molecularrecognition devices are described in Grate et al., Sensors and ActuatorsB, 3, 85–111 (1991) and Grate et al., Analytical Chemistry, Vol. 65, No.14, Jul. 15, 1993, both of which are incorporated herein by reference.

Frequently, the substrate is a piezoelectric material or a waveguide,which can detect small changes in mass. One illustrative example of adevice relying upon molecular recognition as a surface is known as asurface acoustic wave (SAW) sensor. SAW devices function by generatingmechanical surface waves on a thin slab of a piezoelectric material,such as quartz, that oscillates at a characteristic resonant frequencywhen placed in a feedback circuit with a radio frequency amplifier. Theoscillator frequency is measurably altered by small changes in massand/or elastic modulus at the surface of the SAW device.

SAW devices can be adapted to a variety of gas-phase analytical problemsby designing or selecting specific coatings for particular applications.The use of chemoselective polymers for chemical sensor application iswell established as a way to increase the sensitivity and selectivity ofa chemical sensor with respect to specific classes or types of analytes.Typically, a chemoselective polymer is designed to contain functionalgroups that can interact preferentially with the target analyte throughdipole-dipole, Van der Waal's, or hydrogen bonding forces. For example,strong hydrogen bond donating characteristics are important for thedetection of species that are hydrogen bond acceptors, such as toxicorganophosphorus compounds. Increasing the density of hydrogen bondacidic binding sites in the coating of a sensor results in an increasein sensitivity.

Chemoselective films or coatings used with chemical sensors have beendescribed by McGill et al. in Chemtech, Vol. 24, No. 9, 27–37 (1994).The materials used as the chemically active, selectively absorbent layerof a molecular recognition device have often been polymers, as describedin Hansani in Polymer Films in Sensor Applications (Technomic,Lancaster, Pa. 1995). For example, Ting et al. investigated polystyrenesubstituted with hexafluoroisopropanol (HFIP) groups for itscompatibility with other polymers in Journal of Polymer Science: PolymerLetters Edition, Vol. 18, 201–209 (1980) Later, Chang et al. and Barlowet al. investigated a similar material for its use as a sorbent fororganophosphorus vapors, and examined its behavior on a bulk quartzcrystal monitor device in Polymer Engineering and Science, Vol. 27, No.10, 693–702 and 703–15 (1987). Snow et al. (NRL Letter Report,6120-884A) and Sprague et al. (Proceedings of the 1987 U.S. ArmyChemical Research Development and Engineering Center ScientificConference on Chemical Defense Research, page 1241) reported makingmaterials containing HFIP that were based on polystyrene andpoly(isoprene) polymer backbones, where the HFIP provided stronghydrogen bond acidic properties. These materials were used as coatingson molecular recognition devices, such as SAW sensors, and showed highsensitivity for organophosphorus vapors. However, both the parentpolymers and the HFIP-containing materials were glassy or crystalline atroom temperature. Because vapor diffusion may be retarded in glassy orcrystalline materials, the sensors produced were slow to respond andrecover. Further, these are polymeric materials and, like all polymers,they can vary significantly from batch to batch in precise composition,purity and yield. Additional information is reported in Polym. Eng. Sci,27, 693 and 703–715 (1987).

Vicari et al., U.S. Pat. No. 6,114,489, issued Sep. 5, 2000, disclosesreactive hyper-branched polymers containing terminal hydroxy, carboxy,epoxy, and isocyanate groups. The compounds are useful as components inpowder coating compositions for the formation of hard, impact resistantfilms. Examples of the preferred hyperbranched polyesters are thoseformed from α,α-bis(hydroxymethyl)-propionic acid. The backbone of thesehyperbranched polymers are composed of polyester units.

Okawa et al., U.S. Pat. No. 6,140,525 issued Oct. 31, 2000, discloses aclass of hyperbranched polymers that are prepared by contactingmacromonomers that have both silicon hydride and unsaturated organicterminal groups with group VIII metal catalysts. The hyperbranchedpolymers are useful as surfactants, gelling agents, drug deliverysystems, and polymeric absorbents. The hyperbranched polymer backbonesare comprised of a combination of siloxane and carbosilane segments.Examples of preferred macro-monomers include(HSi(CH₃)₂O)₂Si(CH₃)OSi(CH₃)₂(CH₂)₂(Si(CH₃)₂O)_(n)Si(CH₃)₂CH═CH₂, wheren is 10 to 100.

Decker et al., U.S. Pat. No. 6,001,945, issued Dec. 14, 2001, discloseshyperbranched polymers containing silicon atoms and a method of makingthese materials. The exchange (condensation) reaction that forms thehyperbranched polymers results in the elimination of an alcoholby-product and the formation of hyperbranched polymer backbonescomprised of siloxane linkages.

The inventors have now discovered a class of hyperbranched moleculesthat can be used to produce hydrogen bond acidic coatings for chemicalsensor applications. Using the hyperbranched molecules that are highlyfunctionalized results in significant sensitivity improvements. Further,the chemoselective hyper-branched molecules of the present inventionexhibit, not only improved sensitivity to organophosphorus species, butalso high selectivity and sensitivity toward nitro-substituted chemicalvapors, and are thus also useful for detecting the presence ofexplosives. Conventional explosives, such as trinitrotoluene (TNT),hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), andoctahydro-1,3,5-trinitro-1,3,5,7-tetrazocine (HMX), may be contained inunexploded munitions, e.g., buried below the surface of the ground. Suchmunitions exude or leak vapors of the explosive. These vapors aretypically concentrated in the surrounding soil and then migrate to thesurface where they can be detected by the compounds, devices and methodsof the invention.

SUMMARY OF THE INVENTION

According to a first aspect of the present invention, there is provideda hyperbranched polymeric compound having; (1) a polymer backboneportion that is at least partly randomly branched; (2) at least onependant group extending from the polymer backbone portion; (3) and atleast one halogen substituted alcohol or phenol group substituted at thependant group(s) of the polymer backbone portion.

According to a second aspect of the invention, there is provided adevice for selective molecular detection, the device comprising asensing portion, wherein the sensing portion includes a substrate havingcoated thereon a layer, the layer comprising the hyperbranched compoundof the invention.

According to another aspect of the invention, there is provided a methodof detecting a hydrogen bond accepting vapor, such as a nitroaromaticvapor, comprising the steps of:

-   -   (a) contacting the molecules of such a vapor with the sensing        portion of the device of the invention;    -   (b) collecting the molecules in the layer of the device, the        molecules altering a specific physical property of the layer;        and    -   (c) detecting the amount of change with respect to the physical        property from before the contacting step (a) and after the        collecting step (b).

According to yet another aspect of the invention, there is provided asolution for preparing a chemical vapor sensor comprising (a) an amountof the hyperbranched compound of the invention effective to enhance thesensitivity of the sensor to hydrogen bond accepting vapors such aschemical agents or nitroaromatic compounds and (b) a solvent for thehyperbranched compound.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows an example of a hyperbranched compound of the invention,here, a hyperbranched polycarbosilane with fluoroalcohol functionalizedallyl groups.

FIG. 2 shows another example of a hyperbranched compound of theinvention, here, a hyperbranched polycarbosilane with fluoroalcoholfunctionalized phenyl and allyl groups.

DETAILED DESCRIPTION OF THE INVENTION

The hyperbranched molecules of the invention are polymeric molecularconstructions having a randomly branched backbone portion with attachedpendant groups. The randomly branched backbone portion of the moleculemay be composed of linear, branched, and dendritic units, which maythemselves be further branched, forming the backbone portion of thehyperbranched polymer molecule. The detailed chemical structure of thehyperbranched backbone may dominate certain polymer physical properties.Hyperbranched polymers may be distinguished from dendritic, branched,and linear polymers in that:

-   -   (a) The degree and distribution of branching in a hyperbranched        polymer is variable and, therefore, the molecular weight of the        hyperbranched materials usually occurs over a broad distribution        while a dendrimer has a precise structure and molecular weight;    -   (b) The chemical synthesis of a hyperbranched polymer may be        carried out in a single step from the starting monomer, whereas        the synthesis of a dendritic polymer requires a multistep        synthetic procedure;    -   (c) Linear polymers are not branched; and    -   (d) Branched polymers are branched in a regular fashion whereas        hyperbranched polymers are branched in a random fashion.

Although not bound by theory, it is believed that the hyperbranchedmorphology offers advantages over linear macromolecules with the same orsimilar repeating units because the randomly branched structure imposesparticular physical properties such as reduced polymer chainentanglement, lower glass transition and melting temperatures andincreased availability of terminal functional groups. These constraints,often including steric crowding, inhibit even very long chains frompacking in their thermodynamically preferred conformations forcrystallization, and thereby lower their melting points due to entropicfactors. The random variation in structure that occurs withhyperbranched materials is also a contributing factor to the bulkproperties of these materials. By controlling the structure of thehyperbranched polymer, for example, with suitable ratios of branchingarm length to average branch multiplicity, the free volume available tothe chain ends can be made relatively large. In this case, a large freevolume at the chain ends may facilitate arm segmental motion, althoughthis effect is often negated by steric crowding at the terminal groups.

The compound of the invention is a hyperbranched polymeric compoundhaving; (1) a polymer backbone portion that is at least partly randomlybranched; (2) at least one pendant groups extending from the polymerbackbone portion; (3) and at least one halogen substituted alcohol orphenol group substituted at the pendant group(s) of the polymer backboneportion. The compound may be entirely organic or organometallic incomposition. Preferred compounds can be represented by the generalformula:

wherein A is the hyperbranched backbone portion of the polymer;

-   -   L and M are independently selected pendant groups of the polymer        backbone;    -   X and Y are independently selected halogen substituted alcohol        or phenol groups;    -   q and r are independently selected and at least 1, preferably        ranging from 1 to about 10; and    -   n is at least 3, preferably ranging from 20 to 100,000.

A, the hyperbranched backbone portion of the compound, may be composedof repeating units consisting of a single atom such as a carbon orsilicon atom; a hydrocarbon moiety; an organometallic fragment orcluster; or a silicon based moiety such as a siloxane, carbosilane, orsilylene moiety; or a combination thereof. Examples of useful “A”backbone repeat units include {Si—(-Z)_(x)}, {C—(-Z)_(x)}, Fe(—C₅H₄Z-)₂,C₆H_(n)(-Z)_(6-n), and the like, wherein Z is a hydrocarbon, silylene,carbosilane, siloxane, or carbosiloxan fragment of 1 to 20 atoms inlength, including but not limited to alkylene, alkenylene, alkynylene,cycloalkylene, cycloalkenylene, arylene, or heterocyclene. Preferably,however, A is {Si-(alkylen-)}, {Si-(arylene-)}, or {Si-(alkenylene-)}.Most preferably, A is {Si[(CH₂)_(x)]} wherein x is 1 to 3.

L and M in the above formula are independently selected pendant groupsthat extend from the hyperbranched backbone portion of the compound. Lor M may be saturated or unsaturated. By “unsaturated” is meant any siteof unsaturation, such as, for example, a double or triple bond or anaromatic ring. L or M may be entirely hydrocarbon or may contain one ormore heteroatoms, such as, for example, Si, N, O, S, F, Cl, Br and thelike, and may contain further branching entities. For example, L or Mmay independently be alkylene, alkenylene, alkynylene, arylene,alkylene-arylene, alkenylene-arylene, alkynylene-arylene,—C-(alkenylene)₃, —Si-(alkenylene-)₃, —N-(alkenylene-)₂, or—SiO-(alkenylene-)₃, where alkenylene is as defined above;—C-[alkylene-Si-(alkenylene)₃]₃, —Si-[alkylene-C-(alkenylene)₃]₃,—Si-[alkylene-Si-(alkenylene)₃]₃, —SiO-[alkylene-Si-(alkenylene)₃]₃,—CO-[alkylene-Si-(alkenylene)₃]₃, —Si-[alkylene-N-(alkenylene)₂]₃, wherealkylene and alkenylene are defined as above; —C-(cycloalkenylene-)₃,—Si-(cycloalkenylene-)₃, and CON-(cycloalkenylene-)₃, wherecycloalkenylene is defined as above;—C-[cycloalkylene-Si-(alkenylene)₃]₃,—Si-[cycloalkylene-C-(alkenylene)₃]₃,—Si-[cycloalkylene-Si-(alkenylene)₃]₃,—SiO-[cycloalkylene-Si-(alkenylene)₃]₃,—CO-[cycloalkylene-Si-(alkenylene)₃]₃,—Si-[cycloalkylene-N-(alkenylene)₂]₃, where cycloalkylene and alkenyleneare defined as above; —C-(arylene-)₃, —Si-(arylene-)₃, and—SiO-(arylene-)₃, where arylene is defined as above;—C-(heterocyclene-)₃, —Si-(heterocyclene-)₃, and —SiO-(heterocyclene-)₃,where heterocyclene is as defined above;—C-[alkylene-Si-(alkylene-arylene)₃]₃,—Si-[alkylene-C-(alkylene-arylene)₃]₃,—Si-[alkylene-Si-(alkylene-arylene)₃]₃,—SiO-[alkylene-Si-(alkylene-arylene)₃]₃,—CO-[alkylene-Si-(alkylene-arylene)₃]₃,—Si-[alkylene-N-(alkylene-arylene)₂]₃, where alkylene or arylene aredefined as above.

Preferably, however, L and M are independently an alkenylene,aklylene-arylene, alkeneylene-arylene, -[alkylene-Si-(alkenylene)₃]₃ oran -[alkylene-Si-(alkylene-arylene)₃]₃ group. Even more preferably, Land M are independently —(CH₂)_(m)—, —(CH═CH—CH₂)—, —[(CH₂)_(m)C₆H₄]—,—[(CH₂)_(m)—S—(CH═CH—CH₂—)₃]₃ or —Si—{(CH₂)_(m)—Si—[—(CH₂)_(n)—C₆H₄—]₃}₃wherein m and n are independently 1 to 6.

The novel compounds of the invention are strongly hydrogen bonddonating. They are useful in a variety of applications, especially as acoating material on chemical sensors. They are very sensitive forhydrogen bond accepting vapors such as organophosphorus andnitro-substituted compounds such as a those in a great number ofwell-known toxic and explosive materials, respectively.

The compounds of the invention can be synthesized by reactinghexafluoroacetone with the parent hyperbranched molecule, comprising ahyperbranched backbone A and a number of pendant unsaturated groups,taking advantage of the reactivity of perfluoroketones with terminallyunsaturated groups, as described by Urry et al., J. Org. Chem, 1968, 33,2302–2310, hereby incorporated by reference. Alternatively, thecompounds of the invention can be synthesized by reactinghexafluoroacetone with the parent hyperbranched molecule, comprising ahyperbranched backbone A and a number of pendant groups containingmetalated sites, followed by protonation, as described by Barbarich eral., J. Am. Chem. Soc., 1999, 121, 4280–4281, hereby incorporated byreference. Two such hyperbranched compounds of the invention are shownin FIGS. 1 and 2. Using known methods (see, for example, Whitmarsh, C.K., Interrante, L. V. Organometallics, 1991, 10, 1336–1344; Uhlig, W. J.Polym. Sci., Part A: Polym. Chem., 1998, 36, 725–735 and Koopman, F.,Frey, H. Macromolecules 1996, 29, 3701–3706.) these compounds aretypically synthesized in moderate to high yield.

Once synthesized, these functionalized hyperbranched compounds can becoated to a controlled film thickness on a substrate, either alone ormixed with a solvent or similarly functionalized molecule. Usefulsubstrates include planar chemical sensors, such as surface acousticwave (SAW) substrates; silica optical fibers; microcantilevers and otherMEMS devices, and the interior surfaces of silica capillaries. Thesubstrate chosen is based on the sensing mechanism being used.

The principle of operation of an acoustic wave device transducerinvolves the production of an acoustic wave that is generated on thesurface or through the bulk of a substrate material and allowed topropagate. To generate an acoustic wave typically requires apiezoelectric material. Applying a time varying electric field to thepiezoelectric material will cause a synchronous mechanical deformationof the substrate with a coincident generation of an acoustic wave in thematerial. The time varying electric field is generated in the surface byapplying a time varying electrical field through one or more electrodes,which are connected to the piezoelectric material via one or more metalwire bonds and to an electrical circuit. Another electrode or electrodesreceives the wave at a distance from the first electrode or electrodes.The second electrode or electrodes is also connected via metal wirebonds to the electrical circuit and the piezoelectric material. Suchdevices are operable in a frequency range of about 2 kilohertz to 10gigahertz, preferably from about 0.2 megahertz to about 2 gigahertz and,more preferably, in the range of between about 200 to 1000 megahertz.

For piezoelectric sensors, piezoelectric substrates well-known in theart, such as ST-cut quartz, are useful in accordance with the invention.In addition to quartz crystals, piezoelectric ceramics, such as those ofthe barium titanate and lead zirconium titanate families, are suitablesubstrates. These include, for example, LiNbO₃; BaTiO₃; 95 wt. %BaTiO₃/5% GaTiO₃; 80 wt. % BaTiO₃/12% PbTiO₃/8% CaTiO₃; PbNb₂O₆;Na_(0.5)K_(0.5)NbO₃; Pb_(0.94)Sr_(0.06)(Ti_(0.48)Sr_(0.52))O₃; andPb_(0.94)(Ti_(0.48)Sr_(0.52))O₃. In some cases, the substrate maycomprise a piezoelectric coating material, such as ZnO or AIN, appliedto a non-piezoelectric material, such as silicon. The piezoelectricproperties of these and other suitable materials are provided in CRCHandbook of Materials Science, Vol. III, Charles T. Lynch, CRC Press:Boca Raton, 198 (1975).

The sensing portion of an acoustic wave device of the invention is thearea under the chemoselective layer where the chemoselective layercovers the transducer. The area of the sensing portion of such a devicecan be on the order of cm² to μm².

An optical waveguide chemical sensor consists of a light source, anoptical waveguide, a chemoselective film or layer, and a detector toanalyze the light after interacting with the layer. The waveguide isused to propagate light to a sensing portion of the device that containsthe chemoselective layer. The light travels towards this coating andinteracts with it. If the analyte being detected is present in thelayer, the optical characteristics of the light may be altered, and thechange is detected by an optically sensitive detector.

Useful optical chemical sensors, commonly referred to as optrodes,typically include light sources such as semiconductor lasers,light-emitting diodes, or halogen lamps; optical waveguides such asfiber optics or planar waveguide substrates; chemoselective layersdeposited on the sensing portion of the optrode exposed to an analyte;and detectors for monitoring the optical characteristics of an optrode.Sorption of the analyte to the chemoselective layer modifies the opticalcharacteristics of the optrode, and this is usually detected as a changein refractive index or light intensity at one or more wavelengths oflight. Thus, for optical sensors, both optical fibers and opticalwave-guides are well-known in the art and useful in the presentinvention.

Fiber optic waveguides for sensor applications are commonly manufacturedfrom silica glass or quartz as the core of the fiber. Surrounding thiscore is a cladding material that exhibits a lower refractive index thanthe core to achieve internal reflectance. Chemoselective layers aretypically applied at the distal tip of a fiber optic or along the sideof the fiber optic where a portion of the cladding material has beenremoved.

Planar waveguide optical sensors use planar substrate devices as lightguides. The use of a planar waveguide normally involves the use ofevanescent wave techniques to take advantage of the large active surfacearea available. Many of these sensors use the fluorescent properties ofa chemoselective layer and are thus called Total Internal ReflectionFluorescence (TIRF) sensors.

Preferably, acoustic wave devices are used as the substrate for thedevice of the invention. Particularly preferred are SAW devices such as915 MHz two-port resonators made of ST-cut quartz with aluminummetallization and a thin silicon dioxide overcoat. SAW resonators andoscillator electronics to drive them are commercially available fromRFM, Dallas, Tex.

Before applying a coating to form the sensor portion of the device ofthe invention, the substrate is usually cleaned. The cleaning proceduretypically involves rinsing the device in an organic solvent and thensubjecting it to plasma cleaning, as is well-known. Optionally, thesubstrate can be silanized with a material such asdiphenyltetramethyldisilazane (DPTMS) by immersing the cleaned substratesurface in liquid DPTMS and then placing the immersed surface into apartially evacuated chamber heated to about 170° C. for about 12 hours.The silanized substrate is then removed and solvent cleaned with, forexample, toluene, methanol, chloroform, or a physical or serialcombination thereof, before applying the chemically sensitive sensorlayer of the device.

The method used for coating the compounds of the invention onto asubstrate is not critical, and various coating methods known in the artmay be used. Typically, the coating is applied to the substrate insolution, either by dipping, spraying or painting, preferably by anairbrush or spin coating process. Laser deposition techniques may alsobe used, particularly when coating MEMS devices. The concentration ofthe compound of the invention in the coating solution should besufficient to provide the viscosity most appropriate for the selectedmethod of coating, and may easily be determined empirically.

The solvent used, although not critical, should be sufficiently volatileas to facilitate quick and easy removal, but not so volatile as tocomplicate the handling of the coating solution prior to being depositedon the substrate. Examples of useful organic solvents include, forexample, hexane, chloroform, dichloromethane, toluene, xylenes,acetonitrile and tetrahydrofuran. J. W. Grate and R. A McGill inAnalytical Chemistry, Vol. 67, No. 21, 4015–19 (1995), the subject ofwhich is hereby incorporated by reference, describe making chemicalacoustic wave detectors by applying a thin film to a surface acousticwave device. The thickness of the chemoselective layer preferably doesnot exceed that which would reduce the frequency of a chemical sensoroperating at 250 megahertz by about 250 kilohertz and, typically, is inth range of about 0.5 nm to 10 microns, preferably in the range of 5 to500 nm.

The coating may comprise a single layer or multiple layers. Withmultiple layers, a layer containing the compound of the invention may becombined with at least one other layer that provides pores suitable forphysically eliminating some chemical species of large size that are notto be monitored.

The process of sorption plays a key role in the performance of chemicalsensors for gas phase analysis. For example, microsensors, which consistof a physical transducer and a selective sorbent layer, sense changes inthe physical properties, such as mass, of the sorbent layer on thesurface of the transducer, due to the sorption of analyte molecules fromthe gas phase into the sorbent layer. Coating properties that are knownto elicit a detectable SAW sensor response are mass (i.e., as determinedby the thickness and density of the coating), elasticity,viscoelasticity, conductivity, and dielectric constant. Changes in theseproperties can also result in changes in the attenuation (i.e., loss ofacoustic power) of the wave. In some situations, monitoring theattenuation may be preferable to monitoring the velocity of a wave.Alternatively, there are some situations where simultaneously monitoringboth velocity and attenuation can be useful. In any event, it is themodification of the sensed properties of the sorbent layer, as a resultof sorption, that results in the detection of analyte molecules in thegas phase. SAW devices coated with compounds of the invention arecapable of detecting mass changes as low as about 100 pg/mm². The vapordiffusion rate into and out of the polymer film is generally rapid, butdoes depend upon the thickness of the polymer film.

Sensor selectivity, the ability to detect a chemical species in anenvironment containing other chemical species, is generally determinedby the ability of the coated layer to specifically sorb the species tobe detected to the exclusion of almost all others. For most coatings,selectivity is obtained based on providing stronger chemicalinteractions between the coated layer and the target species than occursbetween the layer and species that are not to be detected. The method ofselectively detecting the presence of a chemical entity within anenvironment comprises (a) placing the sensing portion of the device ofthe invention in the environment and (b) detecting changes in the coatedlayer of the sensing portion of the device. The environment may begaseous or liquid.

More than one device may be provided. For example, a plurality of sensorportions could be used in a sensor array with, e.g., associated controldevices and software, in a manner similar to conventional proceduresemploying sensor arrays.

After an initial sensing has taken place, the coated sensor layer can bepurged or cleaned by a second stream, allowing the sensing of a newthird stream to take place. For example, for liquid sensingapplications, water- or acid-base solutions could be used as purging orcleaning solutions, depending upon the species being detected and thenature of the layer. For gas applications, dry nitrogen or clean aircould be used as a cleaning stream.

In the devices and methods of the invention, the compounds are goodsorbents for basic vapors, such as organophosphorus andnitro-substituted compounds. It is expected that the devices of theinvention could weigh about 0.25 to 5 pounds and could, therefore, beeasily mounted on a remote or robotic vehicle for automaticallydetecting toxic chemicals or buried explosives or munitions.Alternatively, such a device would also be useful for remotely detectingexplosives vapors emitting from a person intending the destruction ofprivate property and/or personnel, such as, for example, at crowdedpublic places like airports or arenas where terrorist activity may besuspected.

If desired, it is possible to increase the concentration of explosivevapors contained in the area being monitored, i.e., speed up theirrelease from buried or otherwise hidden munitions or explosives, byirradiating the area with electromagnetic radiation. For example, abeam-forming antenna could be employed to direct high frequency to longwavelength microwave radiation at the area suspected of containingburied munitions, such as landmines. This will gently warm the areabeing checked and increase explosive vapor leakage prior to testing withthe device of the invention. Increasing the concentration of vapor inthe soil or other environment surrounding a munition will produce astronger signal following the reaction with the sensor portion of thedevice of the invention.

The chemoselective, hyperbranched compounds of the invention exhibithigh selectivity and sensitivity toward hydrogen bond basic vapors, dueto the sensitivity and selectivity of the halogen substituted alcohol orphenol functional groups that are present. The functionalizedhyperbranched compounds of the invention also have the advantage ofhigh-yield preparation methods, ready purification, in addition tohaving an increased availability of functional groups to analytes, ascompared with linear polymeric coatings. Moreover, the flexibility inthe synthesis of these materials allows one to tailor a wide variety ofrelated chemoselective hyperbranched compounds.

EXAMPLES

Unless otherwise noted, all synthetic procedures were carried out underinert atmosphere using standard Schlenk and vacuum line techniques.Solvents were dried and degassed under an argon atmosphere usingappropriate drying agents.

These examples are intended to illustrate the present invention to thoseskilled in the art and should not be interpreted as limiting the scopeof the invention set forth in the claims.

Example 1 Preparation of [—CH₂—Si(CH═₂CH₂CH₂C(CF₃)₂OH)₂—]_(n)

To a 500 mL flask containing 2.1 g of Mg chips was added 30 mL offreshly distilled THF. The resulting mixture was cooled to 0° C. andtreated with 10 mL (14.65 g) of ClCH₂SiCl₃ via syringe. The reactionmixture was stirred at 0° C. for four hours with an additional 60 mL ofTHF being added in portions as needed to keep the solution from gettingtoo thick due to salt formation. The reaction mixture was then stirredfor 2 hours at room temperature and treated dropwise with allylmagnesiumbromide (162 mL of 1.0 M in ether) over a two hour period. The resultingsolution was stirred at room temperature for 20 hours. The reaction wasthen quenched with saturated aqueous NH₄Cl and the organic portionextracted with diethyl ether, dried over MgSO₄, and filtered through 1cm of SiO₂. Removal of the volatiles left the product polymer as a paleyellow, viscous oil. A sample of the parent polymer (2.0 g) wasdissolved in CHCl₃ (30 mL) and placed into a mild steel cylinder alongwith a magnetic stir bar. The steel cylinder was then cooled in liquidnitrogen and evacuated. Hexafluoroacetone (˜±4.0 g) was introduced intothe steel cylinder via vacuum transfer. The cylinder was sealed, removedfrom the vacuum line, and heated to 65° C. for 48 hours. The cylinderwas then cooled to room temperature and the volatiles removed undervacuum. Once evacuated, the reaction cylinder was opened to the air, andthe hyperbranched compound inside was extracted with chloroform (4×30mL). The resulting solution was filtered through Celite and thevolatiles removed to give a pale brown polymer. FTIR (NaCl, cm⁻¹) showedthe characteristic OH stretch (˜3510 cm⁻¹) verifying the presence of the—C(CF₃)₂OH groups in the functionalized product.

Example 2 Preparation of [—CH₂—Si{CH₂CH₂CH₂C₆H₃(C(CF₃)₂OH)₂}₂]_(n)

To a 500 mL flask containing 7.0 g of Mg chips was added 20 mL offreshly distilled THF. The resulting mixture was cooled to 0° C. andtreated with 10 mL (14.65 g) of ClCH₂SiCl₃ via syringe. The reactionmixture was stirred at 0° C. for four hours with an additional 60 mL ofTHF being added in portions as needed to keep the solution from gettingtoo thick due to salt formation. The reaction mixture was stirred for 2hours at room temperature then cooled to 0° C. and treated drop-wisewith a THF (60 mL) solution of (3-bromopropyl)benzene (25.5 mL) over atwo hour period. The reaction mixture was then allowed to warm withoccasional cooling to maintain the temperature below 40° C. Theresulting solution was stirred at room temperature for 20 hours. Thereaction was then quenched with saturated aqueous NH₄Cl and the organicportion extracted with diethyl ether, dried over MgSO₄, and filteredthrough 1 cm of SiO₂. Removal of the volatiles left the product polymeras a pale yellow, viscous oil. A sample of the parent polymer (2.0 g)was mixed with a catalytic amount of AlCl₃ (0.1 g) and placed into amild steel cylinder along with a magnetic stir bar. The steel cylinderwas then cooled in liquid nitrogen and evacuated. Hexafluoroacetone(˜4.0 g) was introduced into the steel cylinder via vacuum transfer. Thecylinder was sealed, removed from the vacuum line, and heated to 65° C.for 48 hours. The cylinder was then cooled to room temperature and thevolatiles removed under vacuum. Once evacuated, the reaction cylinderwas opened to the air, and the hyperbranched compound inside wasextracted with chloroform (4×30 mL). The resulting solution was washedwith water, dried over MgSO₄, filtered through Celite and the volatilesremoved to give a pale brown viscous oil. FTIR (NaCl, cm⁻¹) showed an OHstretch (˜3510 cm⁻¹) verifying the presence of the —C(CF₃)₂OH groups inthe functionalized product.

Example 3 Preparation of [—CH₂—Si{CH₂CH═CHC₆H₃(C(CF₃)₂OH)₂}₂]_(n)

To a 500 mL flask containing 7.0 g of Mg chips was added 20 mL offreshly distilled THF. The resulting mixture was cooled to 0° C. andtreated with 10 mL (14.65 g) of ClCH₂SiCl₃ via syringe. The reactionmixture was stirred at 0° C. for four hours with an additional 60 mL ofTHF being added in portions as needed to keep the solution from gettingtoo thick due to salt formation. The reaction mixture was stirred for 2hours at room temperature then cooled to 0° C. and treated dropwise witha THF (60 mL) solution of cinnamyl bromide (33.0 g) over a six hourperiod. The resulting solution was stirred at room temperature for 20hours. The reaction was then quenched with saturated aqueous NH₄Cl andthe organic portion extracted with diethyl ether, dried over MgSO₄, andfiltered through 1 cm of SiO₂. Removal of the volatiles left the productpolymer as a yellow, viscous oil. A sample of the parent polymer (2.0 g)was mixed with a catalytic amount of AlCl₃ (0.1 g) and placed into amild steel cylinder along with a magnetic stir bar. The steel cylinderwas then cooled in liquid nitrogen and evacuated. Hexafluoroacetone(˜4.5 g) was introduced into the steel cylinder via vacuum transfer. Thecylinder was sealed, removed from the vacuum line, and heated to 65° C.for 48 hours. The cylinder was then cooled to room temperature and thevolatiles removed under vacuum. Once evacuated, the reaction cylinderwas opened to the air, and the hyperbranched compound inside wasextracted with chloroform (4×30 mL). The resulting solution was washedwith water, dried over MgSO₄, filtered through Celite and the volatilesremoved to give a pale brown viscous oil. FTIR (NaCl, cm⁻¹) showed an OHstretch (˜3510 cm⁻¹) verifying the presence of the —C(CF₃)₂OH groups inthe functionalized product.

Example 4 Preparation of [—(CH₂)₂—Si{CH₂Si{CH═CHCH₂(C(CF₃)₂OH)}₃}₂]_(n)

To a 500 mL flask containing 6.0 g of Mg chips was added 20 mL offreshly distilled THF. The resulting mixture was cooled to 0° C. andtreated with 10.0 mL (16.69 g) of BrCH₂CH₂SiCl₃ via syringe. Thereaction mixture was stirred at 0° C. for four hours with an additional60 mL of THF being added in portions as needed to keep the solution fromgetting too thick due to salt formation. The reaction mixture wasstirred for 2 hours at room temperature then cooled to 0° C. and treateddropwise with a THF (50 mL) solution of chloromethyltriallylsilane (26.0g) over a four hour period. The resulting solution was stirred at roomtemperature for 24 hours. The reaction was then quenched with saturatedaqueous NH₄Cl and the organic portion extracted with diethyl ether,dried over MgSO₄, and filtered through 1 cm of SiO₂. Removal of thevolatiles left the product polymer as a yellow, viscous oil. A sample ofthe parent polymer (2.0 g) was dissolved in CHCl₃ (30 mL) and placedinto a mild steel cylinder along with a magnetic stir-bar. The steelcylinder was then cooled in liquid nitrogen and evacuated.Hexafluoroacetone (˜4.5 g) was introduced into the steel cylinder viavacuum transfer. The cylinder was sealed, removed from the vacuum line,and heated to 65° C. for 48 hours. The cylinder was then cooled to roomtemperature and the volatiles removed under vacuum. Once evacuated, thereaction cylinder was opened to the air, and the hyperbranched compoundinside was extracted with chloroform (4×30 mL). The resulting solutionwas washed with water, dried over MgSO₄, filtered through Celite and thevolatiles removed to give a pale brown viscous oil. FTIR (NaCl, cm⁻¹)showed an OH stretch (˜3510 cm⁻¹) verifying the presence of the—C(CF₃)₂OH groups in the functionalized product.

Example 5 Preparation of [—(CH₂)₃—Si(CH═CHCH₂C(CF₃)₂OH)₂—]_(n)

To a 50 mL flask was added triallylsilane (2.1 g) and tetraallylsilane(0.1 g) along with 1–2 drops of 0.1 mM H₂PtCl₆(H₂O)_(x) in THF. Theresulting mixture was stirred at 50° C. for 18 hours resulting in aviscous pale yellow oil. The reaction mixture was dissolved in 20 mL ofhexanes and filtered through 1 cm of SiO₂. Removal of the volatiles leftthe product polymer as a pale yellow, viscous oil. A sample of theparent polymer (1.0 g) was dissolved in CHCl₃ (20 mL) and placed into amild steel cylinder along with a magnetic stir bar. The steel cylinderwas then cooled in liquid nitrogen and evacuated. Hexafluoroacetone(˜3.0 g) was introduced into the steel cylinder via vacuum transfer. Thecylinder was sealed, removed from the vacuum line, and heated to 65° C.for 48 hours. The cylinder was then cooled to room temperature and thevolatiles removed under vacuum. Once evacuated, the reaction cylinderwas opened to the air, and the hyperbranched compound inside wasextracted with chloroform (4×30 mL). The resulting solution was filteredthrough Celite and the volatiles removed to give a pale brown polymer.FTIR (NaCl, cm⁻¹) showed the characteristic OH stretch (˜3510 cm⁻¹)verifying the presence of the —C(CF₃)₂OH groups in the functionalizedproduct.

Example 6 Preparation of [—(CH₂)₃—Si(CH₂CH₂CH₂C₆H₃(C(CF₃)₂OH)₂)(CH═CHCH₂C(CF₃)₂OH)—]_(n)

To a 50 mL flask was added 3-phenylpropyldiallylsilane (3.1 g) andallylbenzene (0.05 g) along with 1–2 drops of 0.1 mM H₂PtCl₆(H₂O)_(x) inTHF. The resulting mixture was stirred at 50° C. for 22 hours resultingin a viscous pale yellow oil. The reaction mixture was dissolved in 20mL of hexanes and filtered through 1 cm of SiO₂. Removal of thevolatiles left the product polymer as a pale yellow, viscous oil. Asample of the parent polymer (1.5 g) was dissolved in CHCl₃ (30 mL) andplaced into a mild steel cylinder along with a magnetic stir bar. Thesteel cylinder was then cooled in liquid nitrogen and evacuated.Hexafluoroacetone (˜3.5 g) was introduced into the steel cylinder viavacuum transfer. The cylinder was sealed, removed from the vacuum line,and heated to 65° C. for 48 hours. The cylinder was then cooled to roomtemperature and the volatiles removed under vacuum. Once evacuated, thereaction cylinder was opened to the air, and the hyperbranched compoundinside was extracted with chloroform (4×30 mL). The resulting solutionwas filtered through Celite and the volatiles removed to give a palebrown polymer. FTIR (NaCl, cm⁻¹) showed the characteristic OH stretch(˜3510 cm⁻¹) verifying the presence of the —C(CF₃)₂OH groups in thefunctionalized product.

Example 7 Preparation ofco-[-(CH₂)₃—Si(CH₂CH₂CH₂C₆H₃(C(CF₃)₂OH)₂)—]_(m)—[—(CH₂)₃Si(CH═CHCH₂C—(CF₃)₂OH)₂—]_(n)

To a 50 mL flask was added 3-phenylpropyldiallylsilane (1.8 g),triallylsilane (2.5 g) and tetraallylsilane (0.1 g) along with 1–2 dropsof 0.1 mM H₂PtCl₆(H₂O)_(x) in THF. The resulting mixture was stirred at50° C. for 20 hours resulting in a viscous pale yellow oil. The reactionmixture was dissolved in 20 mL of hexanes and filtered through 1 cm ofSiO₂. Removal of the volatiles left the product polymer as a paleyellow, viscous oil. A sample of the parent polymer (1.5 g) wasdissolved in CHCl₃ (30 mL) and placed into a mild steel cylinder alongwith a magnetic stir bar. The steel cylinder was then cooled in liquidnitrogen and evacuated. Hexafluoroacetone (˜4.0 g) was introduced intothe steel cylinder via vacuum transfer. The cylinder was sealed, removedfrom the vacuum line, and heated to 65° C. for 48 hours. The cylinderwas then cooled to room temperature and the volatiles removed undervacuum. Once evacuated, the reaction cylinder was opened to the air, andthe hyperbranched compound inside was extracted with chloroform (4×30mL). The resulting solution was filtered through Celite and thevolatiles removed to give a pale brown polymer. FTIR (NaCl, cm⁻¹) showedthe characteristic OH stretch (˜3510 cm⁻¹) verifying the presence of the—C(CF₃)₂OH groups in the functionalized product.

Example 8 Preparation of co-[Si{CH₂CH₂CH₂Si(CH₂CH₂CH₂C₆H₃(C(CF₃)₂OH)₂)[CH₂CH₂CH₂Si(CH₂CH₂CH₂C₆H₃(C(CF₃)₂OH)₂)(CH═CHCH₂C(CF₃)₂OH)_(2-x)(CH₂CH₂CH₂)_(x)—]₂}₄]—[—(CH₂)₃—Si(CH═CHCH₂C(CF₃)₂OH)₂—]_(n)

To a 100 mL flask was added triallylsilane (2.5 g) and the dendrimericpolymer Si{CH₂CH₂CH₂Si(CH₂CH₂CH₂C₆H₅)[CH₂CH₂CH₂Si(CH₂CH₂CH₂C₆H₅)(CH₂CH═CH₂)₂]₂}₄₋, (0.20 g) along with 1–2drops of 0.1 mM H₂PtCl₆(H₂O)_(x) in THF. The resulting mixture wasstirred at 50° C. for 24 hours resulting in a viscous pale yellow oil.The reaction mixture was dissolved in 20 mL of hexanes and filteredthrough 1 cm of SiO₂. Removal of the volatiles left the product polymeras a pale yellow, viscous oil. A sample of the parent polymer (1.0 g)was dissolved in CHCl₃ (30 mL) and placed into a mild steel cylinderalong with a magnetic stir bar. The steel cylinder was then cooled inliquid nitrogen and evacuated. Hexafluoroacetone (˜3.0 g) was introducedinto the steel cylinder via vacuum transfer. The cylinder was sealed,removed from the vacuum line, and heated to 65° C. for 48 hours. Thecylinder was then cooled to room temperature and the volatiles removedunder vacuum. Once evacuated, the reaction cylinder was opened to theair, and the hyperbranched compound inside was extracted with chloroform(4×30 mL). The resulting solution was filtered through Celite and thevolatiles removed to give a pale brown polymer. FTIR (NaCl, cm⁻¹) showedthe characteristic OH stretch (˜3510 cm⁻¹) verifying the presence of the—C(CF₃)₂OH groups in the functionalized product.

Example 9 Preparation ofco-[-(CH₂)₃—Si(Me)O—]_(m)—[—(CH₂)₃—Si(CH═CHCH₂C(CF₃)₂OH)₂—]_(n)

To a 100 mL flask was added triallylsilane (3.0 g), tetraallylsilane(0.05 g) and poly(methylhydridosiloxane) (0.15 g) along with 1–2 dropsof 0.1 mM H₂PtCl₆(H₂O)_(x) in THF. The resulting mixture was stirred at50° C. for 24 hours resulting in a viscous pale yellow oil. The reactionmixture was dissolved in 20 mL of hexanes and filtered through 1 cm ofSiO₂. Removal of the volatiles left the product polymer as a paleyellow, viscous oil. A sample of the parent polymer (1.0 g) wasdissolved in CHCl₃ (30 mL) and placed into a mild steel cylinder alongwith a magnetic stir bar. The steel cylinder was then cooled in liquidnitrogen and evacuated. Hexafluoroacetone (˜3.0 g) was introduced intothe steel cylinder via vacuum transfer. The cylinder was sealed, removedfrom the vacuum line, and heated to 65° C. for 48 hours. The cylinderwas then cooled to room temperature and the volatiles removed undervacuum. Once evacuated, the reaction cylinder was opened to the air, andthe hyperbranched compound inside was extracted with chloroform (4×30mL). The resulting solution was filtered through Celite and thevolatiles removed to give a pale brown polymer. FTIR (NaCl, cm⁻¹) showedthe characteristic OH stretch (˜3510 cm⁻¹) verifying the presence of the—C(CF₃)₂OH groups in the functionalized product.

Example 10 Applying a Thin Film to a SAW Device

SAW devices are cleaned in a Harrick plasma cleaner prior to polymerfilm application. Spray-coated films of the compound of FIG. 1 inchloroform (1% by weight) are applied to a SAW device using an airbrushsupplied with compressed dry nitrogen. The frequency change of the SAWdevice operating in an oscillator circuit is monitored duringdeposition, using the change in frequency, typically about 250 kHz, as ameasure of the amount of material applied. After application, the filmsare optionally annealed in an oven at 50° C. overnight. Spray-coatedfilms are examined by optical microscopy with a Nikon microscope usingreflected light Nomarski differential interference contrast.

Example 11 Detection of Basic Vapors with a Compound-Coated SAW Device

The compounds of FIGS. 1 and 2 are separately applied to SAW devices andtested against organic vapors at various concentrations. Upon exposureto a vapor, the coated acoustic wave devices undergo a shift infrequency that is proportional to the concentration of the vapor. Timesto steady state response, corresponding to equilibrium partitioning ofthe vapor into the compound layer, are typically under 10 seconds usinga vapor delivery system. From frequency shift data for a vapor atmultiple concentrations, calibration curves are constructed. Thecalibration curves are generally linear at moderate concentrations, butdeviate from linearity at the high and low concentration levels. Linearcalibration curves are consistent with hydrogen-bonding interactions ata finite number of sites in the compound.

Example 12 Coating a Capillary Column

A solution of the compound of FIG. 2 in chloroform is used to coat theinterior surface of several one-meter silica capillary columns with aninside diameter of 100 microns. The procedure to coat a 100-micron i.d.column from Fused Silica Intermediate Polarity (part number 2-5745,Supelco, Pa.) involves filling the capillary with a solution of thecompound, closing one end of the capillary, and pulling a vacuum off theother end of the capillary at a fixed temperature. The solution-filledcolumn is placed into a gas chromatographic oven stabilized at 30° C. tocontrol the temperature. A vacuum is then pulled using an oil-freeTeflon-coated diaphragm pump (Fisher part number 13-875-217C), with avacuum of −70 kPa, typically being applied for about 15–20 hours.

The thickness and thickness uniformity are verified by cutting a coatedcolumn into several pieces and looking at the cross sections using ahigh power optical microscope. The thickness of one micron is usually ingood agreement with the theoretical film thicknesses.

Example 13 Optical Fiber Drawing and Cladding

The compound of FIG. 2 is combined with a solvent to form a viscousmixture, which is stirred until well-blended and degassed under vacuum.The viscous mixture is applied to a fused silica fiber as it is freshlydrawn from a Heathway fiber drawing apparatus through a 2–5 mm Sandcliffcladding cup, and into a 45 cm long clamshell furnace for curing. Theviscous mixture is supplied to the cladding cup under a pressure ofabout 0.8 to about 1.5 psi. The optimal furnace temperature and fiberdraw-speed are typically about 520° C. and 8–9 m/min. respectively.These relatively slow draw rates are usually used for manual control ofthe drawing conditions, but sometimes result in variable core diametersand coating thickness. However, when used with the other conditionsdescribed, a fairly uniform coating that is light yellow in color andslightly tacky to the touch is usually obtained. As the viscosity of thesolution of the compound increases during the fiber drawing, thedelivery pressure should be increased over the course of filling,usually about two hours.

Half-meter to one-meter sections are hand selected for quality. The bestfiber sections made under these conditions have a smooth coating ofabout 25 microns thick over a 180-micron diameter core. All are usuallyeffective in guiding light.

Additional advantages and modifications will readily occur to thoseskilled in the art. Therefore, the invention in its broader aspects isnot limited to the specific details and representative embodiments shownand described herein. Accordingly, various modifications may be madewithout departing from the spirit or scope of the general inventiveconcept as defined by the appended claims and their equivalents.

1. A device for selective molecular recognition, said device comprisinga sensing portion, wherein said sensing portion includes a substratehaving coated thereon a layer, said layer comprising a hyperbranchedcompound having: (1) a polymer backbone portion that is at least partlyrandomly branched; (2) at least one pendant group extending from thebackbone portion; and (3) at least one halogen substituted alcohol orphenol group substituted at the pendant group(s) of the polymerbackbone.
 2. The device of claim 1 wherein said substrate is a surfaceacoustic wave (SAW) substrate.
 3. The device of claim 1 wherein saidcompound has the general formula:

wherein A is the hyperbranched backbone portion of the polymer; L and Mare independently selected pendant groups of said polymer backbone; Xand Y are independently selected halogen substituted alcohol or phenolgroups; q and r are at least 1 and independently selected; and n is atleast
 3. 4. The device of claim 3 wherein A is composed of unitsselected from the group consisting of silicon atoms, carbon atoms,siloxane, carbosilane, silylene moieties, or a combination thereof. 5.The device of claim 3 wherein A is composed of units selected from thegroup consisting of Si-alkylene, Si-arylene, and —Si-alkenylene.
 6. Thedevice of claim 3 wherein: A is selected from the group consisting of—Si—(CH₂)_(n)—, where n=1–3; —Si—(CH(CH₂C₆H₅))—; andSi—(CH₂(C═CH₂)CH₂)—; L and M are independently selected allyl orpropylenephenylene groups; and X and Y are hexafluoroisopropanol groups.7. The device of claim 3 wherein L and M are independently selected fromthe group consisting of -alkylene-Si-(alkenylene)₃ and-alkylene-Si-(alkylene-arylene)₃.
 8. The device of claim 1 wherein saidlayer is deposited on said substrate by a laser-based coating technique.9. A method of detecting the molecules of a hydrogen bond acceptingvapor comprising the steps of: (a) contacting the molecules of saidvapor with a device comprising a sensing portion, wherein said sensingportion includes a substrate having coated thereon a layer, said layercomprising a hyperbranched compound having: (1) a polymer backboneportion that is at least partly randomly branched; (2) at least onependant group extending from the polymer backbone portion; and (3) atleast one halogen substituted alcohol or phenol group substituted at thependant group(s) of the polymer backbone portion, (b) collecting saidmolecules on said layer, wherein said molecules alter a specificphysical property of said layer; and (c) detecting the amount of changein said physical property from before said contacting step (a) and aftersaid collecting step (b).
 10. The method of claim 9 wherein saidsubstrate is a surface acoustic wave (SAW) substrate.
 11. The method ofclaim 9 wherein said compound has the general formula:

wherein A is the hyperbranched backbone portion of the polymer; L and Mare independently selected pendant groups of said polymer backbone; Xand Y are independently selected halogen substituted alcohol or phenolgroups; q and r are at least 1 and independently selected; and n is atleast
 3. 12. The method of claim 11 wherein A is composed of unitsselected from the group consisting of silicon atoms, carbon atoms,siloxane, carbosilane, silylene moieties, and combinations thereof. 13.The method of claim 11 wherein A is composed of units selected from thegroup consisting of Si-alkylene, Si-arylene, or —Si-alkenylene.
 14. Themethod of claim 11 wherein: A is selected from the group consisting of—Si—(CH₂)_(n)—, where n=1–3; —Si—(CH(CH₂C₆H₅))—; andSi—(CH₂(C═CH₂)CH₂)—; L and M are independently selected allyl orpropylenephenylene groups; and X and Y are hexafluoroisopropanol groups.15. The method of claim 11 wherein L and M are independently selectedfrom the group consisting of -alkylene-Si-(alkenylene)₃ and-alkylene-Si-(alkylene-arylene)₃.